By Timothy Richard Parsons
An advent to the quantitative research of seawater, describing intimately organic and chemical suggestions, that are thought of to be among these frequently utilized by organic oceanographers. The handbook presents entire directions for the addition of reagents and calculation of effects with regard fabric for every procedure in order that the unique texts will be consulted if helpful. usually, the ideas require at the least past expert education and techniques wanting very dear apparatus were refrained from
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Additional info for A Manual of Chemical & Biological Methods for Seawater Analysis
Correct the extinction with the reagent blank (and turbidity blank if necessary) and calculate the phosphate concentration as: /ig-at P/l = corrected extinction x F where F is the factor as described below. G. Determination of blank Use distilled water in place of a sample and carry out steps 1-4 above to obtain the extinction of the reagent blank. 02 on a 10-cm cell. //. 816 g of anhydrous potassium dihydrogen phosphate, KH2PO4, in 1 1 of distilled water. Store in a dark bottle with 1 ml of chloroform; the solution is stable for many months.
Determination of Phosphate Introduction The procedure given below is taken from M u r p h y and Riley (Anal. Acta, 27: 31, 1962). Chim. Method A. 03/n> /zg-at/1 B. Outline of method The seawater sample is allowed to react with a composite reagent containing molybdic acid, ascorbic acid and trivalent antimony. The resulting complex is reduced to give a blue solution which is measured at 885 nm. C. Special apparatus and equipment 130-ml capacity screw-capped polyethylene bottles marked on the side with 100 ml volume with black tape, or Erlenmeyer 125-ml graduated flasks.
6: 27, 978). 1N N a O H ) overnight, dipped in tap water, washed with redistilled water, dried in an oven and capped immediately after drying. (b) With the glycine standard solution, the fluorescent intensity reaches a maximum 80 to 140 sec after mixing of the sample and reagent. 5% per min. The periods corresponding to maximum fluorescence vary slightly depending on the species of amino acid. (c) Some caution is required to reduce the background fluorescent intensities. These include complete aging of resin, use of highly pure reagents, and the avoidance of contamination from fingerprints on containers or from air-borne particles in the laboratory.
A Manual of Chemical & Biological Methods for Seawater Analysis by Timothy Richard Parsons