By C.E.R. Jones (Eds.)
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In the proposed HRMS/CAMS/COM system (Figure 2) a second double-focussing MS will be used b e c a u s e it has the capability to focus ions of the same mass which have substantially different translational e n e r g i e s . Collision Proposed Tondem Double-Focussing Mass Spectrometer for Collislonal Activation and Metastable Ion Decomposition Studies Collector fj\ Figure 2. Although our present MS/CAMS/COM system has second generation computer automation for repeated scanning and data acquisition and reduction of the CA s p e c t r a , it is necessary to manually focus the MS separating system (magnetic field) for the next desired component.
The MS separation (MS-I) utilizing a sector magnetic analyzer is limited to a resolving power of ~1 : 1000. Obviously for complex mixtures it would be highly advantageous to separate isobaric multiplets and to determine the elemental composition of the m a s s - s e p a r a t e d components, both of which are w e l l known capabilities of double-focussing high-resolution instruments (HRMS). The present CAMS capability (MS-II) is severely handicapped by resolution limitations, and improved sensitivity would a l s o be welcome.
Curie-point pyrolysis GLC of washed bacterial cells cultured in a complex broth provided the data. The magnitudes of a few peaks from the pyrogram spectra discriminated between the main species of interest (Streptococcus mutans, 5. sanguis, S. mitior). Streptococci of intermediate properties were classified in relation to these species by numerical taxonomic procedures based on the 16 most informative pyrogram peaks. processed into similarity coefficients. The data were Over 1500 analyses were completed on one Chromatographie column containing polyethylene glycol (Carbowax 20M).
Analytical Pyrolysis by C.E.R. Jones (Eds.)