Download PDF by Erika J. Ernst: Antifungal Agents: Methods And Protocols (Methods in

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By Erika J. Ernst

ISBN-10: 1588292770

ISBN-13: 9781588292773

ISBN-10: 1592599435

ISBN-13: 9781592599431

A suite of state of the art molecular equipment for learning antifungal resistance, for locating and comparing either new and latest antifungal medicinal drugs, and for realizing the host reaction and immunotherapy of such brokers. The protocols keep on with the profitable equipment in Molecular drugs™ sequence layout, every one providing step by step laboratory directions, an creation outlining the primary in the back of the strategy, lists of the required apparatus and reagents, and tips about troubleshooting and averting identified pitfalls. Antifungal brokers: tools and Protocols deals clinician-scientists, microbiologists and molecular biologists the efficient instruments they wish at the present time to appreciate and effectively advance new healing brokers for yeast, mildew, and fungal infections.

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Extra info for Antifungal Agents: Methods And Protocols (Methods in Molecular Medicine)

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36, 856–865. 5. Köhler, G. , White, T. , and Agabian, N. (1997) Overexpression of a cloned IMP dehydrogenase gene of Candida albicans confers resistance to the specific inhibitor mycophenolic acid. J. Bacteriol. 179, 2331–2338. 6. , et al. (1999) Host-induced, stage-specific virulence gene activation in Candida albicans during infection. Mol. Microbiol. 32, 533–546. 44 Morschhäuser, Staib, and Köhler 7. , and Morschhäuser, J. (2000) Activation of the multiple drug resistance gene MDR1 in fluconazole-resistant, clinical Candida albicans strains is caused by mutations in a trans-regulatory factor.

Lockhart, S. , Reed, B. , Pierson, C. , and Soll, D. R. (1996). Most frequent scenario for recurrent Candida vaginitis is strain maintenance with “substrain shuffling”: demonstration by sequential DNA fingerprinting with probes Ca3, C1, and CARE2. J. Clin. Microbiol. 34, 767–777. Targeted Gene Deletion in C. albicans 35 4 Targeted Gene Deletion in Candida albicans Wild-Type Strains by MPAR Flipping Joachim Morschhäuser, Peter Staib, and Gerwald Köhler Summary Many genetic and phenotypic changes occur during the development of drug resistance in fungi.

Lockhart, S. , and Soll, D. R. (1997) Parity among the randomly amplified polymorphic DNA method, multilocus enzyme electrophoresis, and Southern blot hybridization with the moderately repetitive DNA probe Ca3 for fingerprinting Candida albicans. J. Clin. Microbiol. 35, 2348–2358. 4. , and Soll, D. R. (1993) Development of DNA probes for fingerprinting Aspergillus fumigatus. J. Clin. Microbiol. 31, 1547–1554. 5. , and Soll, D. R. (1996) Development of two species-specific fingerprinting probes for broad computer-assisted epidemiological studies of Candida tropicalis.

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Antifungal Agents: Methods And Protocols (Methods in Molecular Medicine) by Erika J. Ernst

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